Probability of Maternity =99.9996097410%\mathbf{= 9 9 . 9 9 9 6 0 9 7 4 1 0 \%} 母系关系概率 =99.9996097410%\mathbf{= 9 9 . 9 9 9 6 0 9 7 4 1 0 \%}
The alleged mother is not excluded as the biological mother of the tested child __ Gu, Ariana. 被指称为母亲的个体未被排除为受测儿童__顾·阿里安娜的生物学母亲
Based on testing obtained from analyses of the DNA loci listed, the Probability of Maternity is 99.9996097410%\mathbf{9 9 . 9 9 9 6 0 9 7 4 1 0 \%}. 根据所列 DNA 位点分析检测结果，亲子关系概率为 99.9996097410%\mathbf{9 9 . 9 9 9 6 0 9 7 4 1 0 \%} LL, the undersigned Laboratory Director, verify that the interpretation of the results is correct as reported on quad\quad 09/30/2024. LL ，本实验室主任签字确认，截至 quad\quad 2024 年 9 月 30 日的检测结果解读正确无误
参考等位基因频率数据库：商业 STR 多重检测试剂盒中 29 个常染色体 STR 位点在美国无关人群样本中的等位基因频率值。表格中每个标记下方列出了观测杂合度值(Hobs)、精确检验的哈迪-温伯格平衡 p 值(HWE)、个体识别概率值(PI)以及父权排除概率值(PPE)。
Reference Allele Frequency Database:
Allele frequency values for unrelated U.S. population samples (n=1036)(\mathrm{n}=1036) at 29 autosomal STR loci in commercial STR multiplex kits. The observed heterozygosity values (Hobs), Hardy-Weinberg equilibrium p-value from exact test (HWE), probability of identity values (PI), and probability of paternity exclusion values (PPE) are listed below each marker in the table.
Reference Allele Frequency Database:
Allele frequency values for unrelated U.S. population samples (n=1036) at 29 autosomal STR loci in commercial STR multiplex kits. The observed heterozygosity values (Hobs), Hardy-Weinberg equilibrium p-value from exact test (HWE), probability of identity values (PI), and probability of paternity exclusion values (PPE) are listed below each marker in the table.| Reference Allele Frequency Database: |
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| Allele frequency values for unrelated U.S. population samples $(\mathrm{n}=1036)$ at 29 autosomal STR loci in commercial STR multiplex kits. The observed heterozygosity values (Hobs), Hardy-Weinberg equilibrium p-value from exact test (HWE), probability of identity values (PI), and probability of paternity exclusion values (PPE) are listed below each marker in the table. |
Platform used: Sanger Sequencing 3500 Dx
"Reference Allele Frequency Database:
Allele frequency values for unrelated U.S. population samples (n=1036) at 29 autosomal STR loci in commercial STR multiplex kits. The observed heterozygosity values (Hobs), Hardy-Weinberg equilibrium p-value from exact test (HWE), probability of identity values (PI), and probability of paternity exclusion values (PPE) are listed below each marker in the table."
Genome build used: UCSC hg19 (NCBI Build 37)| Platform used: Sanger Sequencing 3500 Dx |
| :--- |
| Reference Allele Frequency Database: <br> Allele frequency values for unrelated U.S. population samples $(\mathrm{n}=1036)$ at 29 autosomal STR loci in commercial STR multiplex kits. The observed heterozygosity values (Hobs), Hardy-Weinberg equilibrium p-value from exact test (HWE), probability of identity values (PI), and probability of paternity exclusion values (PPE) are listed below each marker in the table. |
| Genome build used: UCSC hg19 (NCBI Build 37) |
STR-based Fatherless Paternity Test is a RUO test developed by PacGenomics. This test was developed and its performance characteristics determined by PacGenomics clinical genetics laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration. 基于 STR 的无父亲亲权检测是由 PacGenomics 开发的仅供研究用(RUO)检测方法。该检测由 PacGenomics 临床遗传学实验室开发并确定其性能特征，尚未获得美国食品药品监督管理局的批准或认证。
This analysis is not considered as routine medical care. 本分析不作为常规医疗服务
Confirmed by 经确认
Man Li, PhD 李曼博士
HCLD (Molecular Diagnostics) 分子诊断学高级临床实验室主任
Certified New York State PGD (Molecular) Director 纽约州认证分子遗传学项目主任（PGD）
Clinical Genetic Molecular Biologist Scientist (CGMBS) 临床遗传分子生物学家（CGMBS）
ACKNOWLEDGMENT 证书
A notary public or other officer completing this certificate verifies only the identity of the individual who signed the document to which this certificate is attached, and not the truthfulness, accuracy, or validity of that document. 公证员或其他签署本证书的官员仅核实所附文件签署人的身份，不对该文件的真实性、准确性或有效性进行验证。
State of California 加利福尼亚州
County of Los Angeles 洛杉矶县
On qquad\qquad 9/30/2024 before me, SHUE GUAN, NOTARY PUBLIC personally appeared MAN LI, who proved to me on the basis of satisfactory evidence to be the person whose name is subscribed to the within instrument and acknowledged to me that she executed the same in her authorized capacity, and that by her signature on the instrument the person, or the entity upon behalf of which the person acted, executed the instrument. 2024 年 9 月 30 日，公证员 SHUE GUAN 亲自见证 MAN LI 出示了令本人信服的身份证件，确认其为文件署名者，并承认其以授权身份签署本文件。通过在本文件上的签名，该人士或其代表的实体已执行本文件。
I certify under PENALTY OF PERJURY under the laws of the State of California that the foregoing paragraph is true and correct. 我谨此根据加利福尼亚州法律以伪证罪处罚为担保，证明前述陈述真实无误。
